Our approach

From designing new chemicals to measuring tissue metabolites and performance

Applied Bioenergetics Lab is carefully furnished with state-of-the-art equipment suitable for investigating and recording a number of biochemical, physiological, psychological and medical outcomes primarily in human participants.

Equipment on- and off-site fueled up by our expertise provides the technology essential to understand (patho)physiology of clinical bioenergetics, but also to monitor a plethora of biomarkers and patient-reported feedbacks in response to specific interventions, thus providing accurate and timely results to clinicians, customers, and for scientific purposes.

We use COSMED CPET metabolic cart to measure metabolic expenditure at rest and during exercise through the measurement of oxygen consumption (VO2) and carbon dioxide production (VCO2). This cutting-edge device is accompanied by 2-s NIRS system for evaluation of metabolic flexibility and oxygen consumption in the skeletal muscles.

1.5 Tesla Siemens Avanto scanner enables qualitative and quantitative imaging of energy metabolism in energy-rich tissues, such as the brain, liver and skeletal muscle, using MR spectroscopy assays.

Off-site biochemical lab provides a plethora of advanced assays for bioenergetics markers in serum, urine and saliva using robust Hewlett-Packard gas chromatography and high-sensitive HPLC devices. Applied Bioenergetics Lab maintains a new biobank of samples stored at – 80 degrees Celsius.

Globus Ergo Tesys system enables real-time evaluation of isodynamic and isotonic muscular performance, using highly-sensitive 200 Hz load cell and T90 dynamic transducer, with both devices synchronized with 4-channel electromyography and photocell gates.

Cinematic evaluation devices include US Probotics jump system, FEI Baseline back-leg-chest and handgrip dynamometers, and Hi-Res 360 Degree goniometer.

HP Cosmos treadmill and FitLine ergometer accompanied by Custo·Med 12-chanell stress ECG with HRV module, deliver technical and performance data on intermittent and continuous stress testing.

We are equipped and trained to evaluate many patient-reported outcomes for ambulatory and in-clinic patients and healthy populations, including: Alzheimer’s Disease Assessment Scale (ADAS-Cog), Mini Mental State Examination (MMSE), Sarcopenia-Specific Quality of Life (SarQoL) and SARC-F Screen for Sarcopenia, SMFA Injury and Arthritis Survey, Visual Analog Scale for Pain (VAS Pain), Impact of Weight on Quality of Life (IWQOL), SF-36 and SF-12 Quality of Life (QoL) surveys, Adverse Event Assessment (AEA) score etc.

To address a possible role of microbiota energy utilization in the gut-brain and gut-muscle axes, we quantify the concentration of gut flora-generated gasses (such as molecular hydrogen) in human breath using electrochemical Fischer Analysen Instrumente system.

Building our expertise in this area, we just started to profile microbiota by targeted 16S rRNA-specific q-PCR with help from subcontracted lab.

Our multidisciplinary team continuously work to develop new nutraceuticals as our own IP, and help industry partners to screen and improve technical characteristics of their own formulas, including better bioavailability, extended shelf life or more advantageous performance.

Approaches used include ionic liquid production technology, amino acid derivatization, or herbal processing and extraction.

We also provide professional help to companies for various legislation issues, including health claim applications, GRAS notification process, and FDA/EFSA communication for dietary supplements and nutraceuticals.